0704-883-0675     |      dataprojectng@gmail.com

THE AMELIORATIVE EFFECT OF ACACIA SIEBERIANA D.C. METHANOL ROOT BARK EXTRACT ON INDUCED LIVER INJURY AND ITS SUBCHRONIC TOXICITY PROFILE IN RATS

  • Project Research
  • 1-5 Chapters
  • Abstract : Available
  • Table of Content: Available
  • Reference Style: APA
  • Recommended for : Student Researchers
  • NGN 3000

ABSTRACT

Acacia sieberiana D.C is traditionally used as a remedy for various ailments including hepatitis. The effect of methanol root bark extract of Acacia sieberiana (ASE) on paracetamol (PCM) - and bile duct ligation (BDL) –induced liver injury wereinvestigated in rats. Rats were divided into six groups (Groups I-VI) of 5 and 6 rats for PCM and BDL, respectively. For the PCM-induced liver injury study, animals were pre-treated with normal saline, silymarin (50mg/kg) and extract (250, 750, 1,500 mg/kg)for 7 days. After the last dose, liver injury was initiated by the administration of PCM (2g/kg). In BDL-induced liver injury study, 6 nonligated but operated rats were used as control (Group I), Groups I and II received normal saline while Groups III-VI received silymarin (50mg/kg), the extract (125, 250 and 380 mg/kg), respectively, for 7 days. At the end of the experiment, blood and organs were obtainedfor biochemical and histological assay. Results showed the presence of carbohydrates, glycosides, triterpenes, cardiac glycosides, saponins, tannins, flavonoids and alkaloids. The LD50 values for oral and intraperitoneal routes of ASE were >5,000 and 1,300 mg/kg respectively. The subchronic toxicity study revealed that ASE significantly (P<0.05) reduced body weight when compared to the control and significantly (P<0.05) elevated ALP, whereas, serum urea and blood lymphocytes were significantly (P<0.05) increased at the 1500mg/kg dose group. The histology of the heart revealed no effect. However, the liver produced a dose-dependent hepatocellular necrosis and vacuolations. There was also lymphocyte hyperplasia and glomerular necrosis of the kidneys and alveolar congestion of the lungs. PCM significantly (P<0.05) elevated ALT, AST, direct Bilirubin and significantly (P<0.05) decreased total protein and albumin when compared to ASE. Whereas, ASE at 250 and 750 mg/kg was seen to significantly (P<0.05) decrease the levels of ALT and AST, while xix total protein and albumin were significantly (P<0.05) elevated in ASE-treated groups. However, direct bilirubin was significantly (P<0.05) decreased in silymarin group and 750 mg/kg ASE group. Furthermore, PCM significantly (P<0.05) decreased SOD and increased MDA when compared with ASE pre-treated groups. The histology revealed, PCM caused severe necrosis of the hepatocytes with vascular and sinusoid congestions but the impacts were mild with silymarin and lower doses of ASE pre-treated groups. The BDL–induced liver injury study revealed that ASE significantly (P<0.05) decreased AST level at lower doses and significantly decreased ALP level at higher doses. Likewise, ASE significantly (P<0.05) decreased direct and total bilirubin levels in silymarin and ASE treated groups. There was significant (P<0.05) elevation of SOD at lower ASE doses while GPx and CAT were significantly (P<0.05) elevated in ASE-treated groups. Furthermore, MDA was only significantly decreased at 125 mg/kg ASE group. Histology revealed that themorphology of liver tissue was preserved at 125 and 250 mg/kg ASE groups from BDL-induced moderate necrosis and vascular congestion. The study concludes that Acacia sieberiana is relatively non-toxic with acute administration and has hepatoprotective potentials on PCM- and BDLinduced liver injury at its lower doses but could be relatively toxic at its higher doses and/with prolonged use.




FIND OTHER RELATED TOPICS


Related Project Materials

SIGNIFICANCE OF EXTERNAL AUDITORS ON THE EXAMINATION OF FINANCIAL STATEMENT

ABSTRACT

This study was to assess the significance external auditor‟s in the examination of financial statement of first Bank of Nigeria...

Read more
EXPLORING THE USES OF SOCIAL MEDIA PLATFORMS AS SUPPLEMENTARY TOOLS FOR PEER SUPPORT AND CONNECTION AMONG INDIVIDUALS WITH SCHIZOPHRENIA IN PSYCHOSOCIAL REHABILITATION

Background of the study

In recent years, the landscape of mental health support has undergone a profoun...

Read more
ATTITUDE OF MOTHERS TOWARDS IMMUNIZATION IN IKPOBA-OKHA LOCAL GOVERNMENT AREA OF EDO STATE

ABSTRACT

This study is attempted to provide a general information and knowledge about the attitude of mothers toward...

Read more
SMARTBIN: EFFICIENT WASTE HANDLING BASED ON THE INTERNET OF THINGS (IOT)

ABSTRACT

In our present society today, it is clearly seen that garbage cans are being placed in open environments because it needs to be...

Read more
NUCLEAR ENERGY PROLIFERATION: LESSONS FOR THE THIRD WORLD NATIONS, 1945-2006

Abstract

The proliferation of nuclear energy is a phenomenon of the 21stcentury ...

Read more
CREDIT MANAGEMENT AND THE INCIDENCE OF BAD DEBT IN NIGERIA MONEY-DEPOSIT BANKS. (A CASE STUDY OF UNION BANK OF NIGERIA PLC)

                         &n...

Read more
THE EXTENT OF IMPLEMENTATION OF SAFETY PRACTICES IN BIOLOGY LABORATORY AMONG SENIOR SECONDARY SCHOOL STUDENTS IN ENUGU SOUTH LOCAL GOVERNMENT AREA

ABSTRACT

 

This study outlays different individual’s conception of science. Closely ra...

Read more
INCIDENCE OF SURGICAL SITE NOSOCOMIAL INFECTIONS IN FEMALE AND MALE SURGICAL WARDS OF AHMADU BELLO UNIVERSITY TEACHING HOSPITAL, ZARIA, NIGERIA

ABSTRACT

Incidence of surgical site nosocomial infections and antibiotics susceptibility profiles of bacterial isolates in Ahmadu Bello U...

Read more
AN ANALYSIS OF DELAY IN ENFORCEMENT OF CONTRACTUAL JUDGMENTS AS IMPEDIMENT TO FOREIGN DIRECT INVESTMENTS (FDI) IN NIGERIA

ABSTRACT

Foreign Direct Investment (FDI) is about economic prosperity and wealth creation of developing economies, (FDI) brings with it c...

Read more
THE INFLUENCE OF TUBERCULOSIS ON ACADEMIC PERFORMANCE OF PRIMARY SCHOOL PUPILS IN ABA NORTH ABIA STATE

ABSTRACT

This study was carried out to evaluate the influence of tuberculosis on academic performa...

Read more
Share this page with your friends




whatsapp